Thermo Fisher Scientific SLC12A1 Polyclonal Antibody, FITC
SLC12A1(NKCC2) 단백질을 인식하는 FITC 결합 토끼 폴리클로날 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫드, 비인간 영장류 반응성. 친화 크로마토그래피로 정제, -20°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 30–60 on NKCC2 protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The sodium-potassium-chloride cotransporter isoform 2 (NKCC2) is kidney-specific and localized on the apical membrane of the thick ascending limb of Henle’s loop and the macula densa. It mediates NaCl resorption with a stoichiometry of 1Na:1K:2Cl and is sensitive to diuretics such as furosemide and bumetanide. Mutations in this gene are associated with Bartter-like syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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