Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), APC, eBioscience

Applications

• Flow Cytometry (Flow): 1 µg/Test
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Product Specifications

Additional Formats

• Unconjugated
• Biotin
• Brilliant UV 737
• PE
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, structurally similar to CD28, is expressed at low levels on activated T cells and binds B7 family members CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28. While CD28 provides a costimulatory signal, CTLA-4 inhibits T cell activation by reducing IL-2 secretion and proliferation. A large portion of CTLA-4 is localized intracellularly due to ER retention motifs, thus intracellular staining may be necessary for complete detection.

Applications Reported:

• Flow cytometric analysis
• Intracellular staining followed by flow cytometry

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Refer to BestProtocols®: Protocol B for intracellular protein staining.

• Recommended usage: ≤1 µg per 1×10⁶ cells in 100 µL total staining volume
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4⁺ T cells. It binds CD80 and CD86 on antigen-presenting cells with higher affinity than CD28, delivering inhibitory signals that suppress T cell activation. Intracellular CTLA4 is also found in regulatory T cells and contributes to their function.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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