Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (MR1), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is transiently expressed by activated T cells and plays a key role in T cell–APC interactions. Binding of CD154 to CD40 on antigen-presenting cells such as B cells, monocytes/macrophages, and dendritic cells induces T-dependent B cell activation and proliferation.

For flow cytometric staining, stimulation of enriched T cells or CD4 cells (via depletion strategy) prior to staining with MR1 is recommended.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry of stimulated mouse splenocytes
• Recommended Usage: ≤0.25 µg per test (100 µL final volume; 10⁵–10⁸ cells/test)
• Excitation/Emission: 633–647 nm / 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein expressed on T cells. It stimulates B cell proliferation and immunoglobulin secretion in the presence of cytokines, induces cytokine production and tumoricidal activity in monocytes, and co-stimulates activated T cell proliferation with IFN-γ, TNF-α, and IL-2 production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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