Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), APC, eBioscience
인간 및 붉은털원숭이 CD152(CTLA-4)를 인식하는 14D3 단클론 항체로 APC 형광체가 결합된 제품입니다. Flow Cytometry용으로 최적화되어 있으며, 고순도 친화 크로마토그래피 정제 및 4°C 보관 권장. 세포 내 및 표면 CTLA-4 분석에 적합합니다.
- 카탈로그번호
- 17-1529-4x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), APC, eBioscience
Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), APC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.03 µg)/test
Publications: View 11 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14D3 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688161 |
Additional Formats
- Unconjugated
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The 14D3 monoclonal antibody reacts with human CD152 (CTLA-4), a protein structurally similar to CD28. CTLA-4 is expressed on activated T cells and binds B7 family members CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 delivers inhibitory signals to T cells, restricting activation by reducing IL-2 secretion and proliferation. The cytoplasmic domain contains ER retention motifs, leading to intracellular localization upon TCR signaling.
This antibody also recognizes rhesus monkey CTLA-4 and exhibits inhibitory activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometry using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and BestProtocols® (Protocol A): Two-step protocol for cytoplasmic intracellular proteins.
Use at 5 µL (0.03 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
For complete detection, assess both intracellular and surface CTLA-4 expression.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed mainly on CD4+ T cells. It binds CD80 and CD86 with higher affinity than CD28, delivering inhibitory signals that prevent T cell activation. Intracellular CTLA4 is also found in regulatory T cells and contributes to their suppressive function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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