Thermo Fisher Scientific CD155 Monoclonal Antibody (TX56), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TX56

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603205

Product Specific Information

Description: This TX56 monoclonal antibody reacts with mouse CD155 (also known as poliovirus receptor or PVR), a member of the Ig superfamily of nectins and nectin-like proteins. CD155 is expressed on thymocytes (e.g. CD4+CD8+ and CD4+ cells), peripheral T and B lymphocytes (e.g. regulatory T cells, activated T cells, marginal zone B cells, and plasma cells), natural killer (NK) cells, dendritic cells (e.g. mature and activated), and monocytes. CD155 interacts with other nectins, including Nectin-3 and the extraceullar matrix protein vitronectin, the adhesion molecule CD226/DNAM-1, and the NK cell receptor CD96. CD155 plays a role in cell-cell adhesion, including formation of adherens junctions, cell migration, polarization, and NK-mediated cytotoxicity. In humans, but not in mice, CD155 is the receptor for poliovirus.

Applications Reported: This TX56 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TX56 antibody has been tested by flow cytometric analysis on mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.