Thermo Fisher Scientific Human Melanocyte Basal Medium Kit, calcium free (formerly Medium 254CF Kit)

Gibco™ Human Melanocyte Basal Medium Kit, Calcium Free (formerly Medium 254CF Kit)

The Gibco Human Melanocyte Basal Medium Kit, Calcium Free (formerly Medium 254CF Kit) enables the culture of human epidermal melanocytes.

구성품

• Human Melanocyte Basal Medium, Calcium Free (formerly Medium 254CF):
  Calcium-free version of Human Melanocyte Basal Medium (formerly Medium 254) for users who wish to vary calcium concentration.
• Sterile stock solution of calcium chloride (0.2 M, 0.5 mL)

제품 설명

Human Melanocyte Basal Medium, Calcium Free, is a basal medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts.
This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins.
It is HEPES and bicarbonate buffered, designed for use in an incubator with an atmosphere of 5% CO₂ / 95% air.

To support plating and long-term proliferation of normal human melanocytes, this medium must be supplemented with calcium plus either:

• Human Melanocyte Growth Supplement (HMGS, Cat. No. S-002-5), or
• PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2, Cat. No. S-016-5)

Each supplement contains all growth factors, hormones, and tissue extracts necessary for melanocyte growth in this medium.

Note: Calcium chloride must be added to this medium prior to use.
Calcium concentration from other sources is 0.5 μM in unsupplemented Human Melanocyte Basal Medium, Calcium Free.

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