Thermo Fisher Scientific IL-4 Monoclonal Antibody (E10022), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

0.001-20 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E10022

Immunogen

Recombinant human IL-4 purified from Pichia pastoris

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Lyophilized

Concentration

0.11 mg/mL

Purification

Protein G

Storage buffer

PBS with 10% trehalose

Contains

proprietary preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_417049

Product Specific Information

M2IL4BI targets IL-4 in ELISA applications and shows reactivity with Human samples.

The M2IL4BI immunogen is recombinant human IL-4 purified from Pichia pastoris.

M2IL4BI detects IL-4 which has a predicted molecular weight of approximately 15 kDa.

The M2IL4BI IL4 antibody (biotinylated conjugate of clone E10022) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M3IL4I (clone E10023). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M2IL4BI (clone E10022) and antibody M3IL4I (clone E10023) have successfully been used in combination with recombinant IL4 protein SIL4 in ELISA applications.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.