Thermo Fisher Scientific CCL5 (RANTES) Monoclonal Antibody (VL-1), Biotin

Applications

Western Blot (WB)

• Assay-dependent

Flow Cytometry (Flow)

• Not specified

ELISA

• Assay-dependent

Product Specifications

Product Specific Information

M230B targets RANTES in ELISA and WB applications and shows reactivity with human samples.
The immunogen is recombinant human RANTES, and M230B detects RANTES with a predicted molecular weight of approximately 8 kDa.
The biotinylated antibody M230B (clone VL-1) has been successfully used as the detection antibody in sandwich ELISA with polyclonal capture antibody P230E.
Typical dilutions for sandwich ELISA include:

• 1 µg/mL for coating
• 0.125–0.25 µg/mL for detection

Biotinylated antibody M230B (clone VL-1) and antibody P230E have been used in combination with recombinant RANTES protein SRNTS in ELISA applications.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are secreted proteins involved in immunoregulatory and inflammatory processes.
CCL5 belongs to the CC subfamily and functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils.
It induces histamine release from basophils and activates eosinophils.
CCL5 is one of the major HIV-suppressive factors produced by CD8+ cells and acts as a natural ligand for CCR5, suppressing replication of HIV-1 R5 strains.
Alternative splicing results in multiple transcript variants encoding different isoforms.
Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.