Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (rTP53/6927)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. p53 upregulates stress response genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 consists of an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain.

Phosphorylation at Ser15 is crucial for activation and stabilization, while phosphorylation at Ser392 is linked to tumor formation. p53 plays roles in cancer suppression, aging, oxidative stress, and neuronal function, including learning and memory. Normally expressed at low levels, p53 is highly expressed in transformed cell lines, contributing to malignancy. Mutations in TP53 disrupt DNA binding and tumor suppressor activity, found both as somatic and germline mutations (e.g., Li-Fraumeni syndrome).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.