Thermo Fisher Scientific p53 (Tumor Suppressor Protein) Monoclonal Antibody (rTP53/3889)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.

Phosphorylation at Ser15 is crucial for p53 activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 also plays roles in aging, oxidative stress, and learning and memory processes. Normally expressed at low levels, p53 is upregulated in transformed cells, contributing to malignancy. Mutations in p53 disrupt DNA binding and tumor suppressor function, and alterations in TP53 are linked to various cancers and Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.