Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (DO-7)

Applications and Tested Dilution

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation. Upon activation, p53 induces expression of genes such as p21^waf1, leading to G1 or G2/M arrest.

Structurally, p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for p53 activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 plays a critical role in preventing proliferation of damaged cells, acting as a natural anticancer mechanism. It is also linked to aging, oxidative stress, and cognitive function through regulation of long-term potentiation (LTP).

Mutations in the TP53 gene are common in many human cancers, leading to loss of DNA-binding ability and tumor suppressor function. Germline mutations can occur in cancer-prone families, such as those with Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.