Thermo Fisher Scientific DMAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C-D(435) T I I D V V G A P L T R N S R(450) of mouse DMAP1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DMAP1,uniProtId:Q9JI44-1,ncbiNodeId:10090,antigenRange:435-450,antigenLength:468,antigenImageFileName:PA1-886_DMAP1_Q9JI44-1_Rabbit.svg,antigenImageFileNamePDP:PA1-886_DMAP1_Q9JI44-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2292767

Product Specific Information

PA1-886 detects DNA methyltransferase 1 associated protein (DMAP1) from human and mouse cells.

PA1-886 has been successfully used in Western blot and Immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~52 kDa band which corresponds to DMAP1 from HeLa cell nuclear extracts.

PA1-886 immunizing peptide corresponds to amino acid residues 435-450 from mouse DMAP1. This sequence is completely conserved in human DMAP1. PA1-886 immunizing peptide (Cat. # PEP-118) is available for use in neutralization and control experiments.

Target Information

DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.