
Thermo Fisher Scientific H4K12ac Recombinant Superclonal Antibody (45HCLC), ChIP-Verified
Recombinant Superclonal™ 항체로, H4K12ac 타겟에 높은 특이성과 감도를 제공합니다. ChIP, WB, ICC/IF 등 다양한 응용에 최적화되어 있으며, 로트 간 일관성을 보장합니다. Human, Mouse 반응성으로 연구용에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 1–5 µg/million cells |
| Peptide Array (Array) | 1:4,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 45HCLC |
| Immunogen | Peptide corresponding to human H4K12ac [aa9–aa18] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2895909 |
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They consist of multiple different recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, while maintaining the consistency of recombinant production.
These antibodies recognize multiple epitope sites, providing higher detection sensitivity for low-abundance targets.
Each lot contains a defined mixture of light and heavy chains, ensuring reproducibility and eliminating biological variability typical of polyclonal antibodies.
This antibody is predicted to react with Rat.
Note: Formerly called “Recombinant polyclonal antibody”, now rebranded as “Recombinant Superclonal™ antibody”. The physical product and performance remain unchanged.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromatin in eukaryotes.
Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
The histone H4 gene is intronless, lacks a polyA tail, and contains a palindromic termination element.
It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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