Thermo Fisher Scientific CXCL16 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Neutralization (Neu)

0.05-0.25 µg/mL

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human CXCL16 Asn49-Pro137 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL16,uniProtId:Q9H2A7-1,ncbiNodeId:9606,antigenRange:49-137,antigenLength:254,antigenImageFileName:PA5-47977_CXCL16_Q9H2A7-1_Goat.svg,antigenImageFileNamePDP:PA5-47977_CXCL16_Q9H2A7-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605469

Product Specific Information

In ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) CXCL16, rm6Ckine, recombinant human (rh) BLC, rhCTACK, rhIL-8, rhPF4, and rhLymphotactin is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.