Takara Mung Bean Nuclease

More Information

Applications

• Double-stranded DNA, RNA, or DNA/RNA blunt ending
• cDNA hairpin loop cleavage

Buffer

Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 5.0), 1000 mM NaCl, 10 mM zinc acetate, and 50% glycerol

Product citations

Kowalski, D., Kroeker, W. D. & Laskowski, M. Mung bean nuclease I. Physical, chemical, and catalytic properties. Biochemistry 15, 4457–63 (1976).

Kroeker, W. D., Kowalski, D. & Laskowski, M. Mung bean nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry 15, 4463–7 (1976).

Mathis, D. J., Elkaim, R., Kédinger, C., Sassone-Corsi, P. & Chambon, P. Specific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units. Proc. Natl. Acad. Sci. U. S. A. 78, 7383–7 (1981).

Sheflin, L. G. & Kowalski, D. Altered DNA conformations detected by mung bean nuclease occur in promoter and terminator regions of supercoiled pBR322 DNA. Nucleic Acids Res. 13, 6137–54 (1985).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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