Thermo Fisher Scientific Phospho-c-Raf (Ser259) Recombinant Rabbit Monoclonal Antibody (SD85-07)
Phospho-c-Raf (Ser259) 인산화 단백질 검출용 재조합 토끼 단클론 항체. Western blot 및 IHC(P)에서 높은 특이성과 재현성 제공. HEK293 발현 시스템 기반으로 제조되어 로트 간 일관성 우수. 연구용으로 세포 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD85-07 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser259 of Human Raf1 (aa 236–285) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809685 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Selected clones are screened to identify optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) acting downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which subsequently activate ERK1 and ERK2. Activated ERKs regulate cell physiology including gene expression, cell cycle, apoptosis, differentiation, and migration.
Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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