Thermo Fisher Scientific Cdc23 Recombinant Rabbit Monoclonal Antibody (SD08-60)
Recombinant rabbit monoclonal antibody targeting Human Cdc23 (aa 521-570). Validated for WB and ICC/IF applications. Offers high specificity, lot-to-lot consistency, and animal origin-free formulation. Supplied as liquid, 1 mg/mL, stored at 4°C or -20°C.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD08-60 |
| Immunogen | Synthetic peptide within Human Cdc23 aa 521–570 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809682 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
Ubiquitination is a key mechanism for targeting short-lived or abnormal proteins for degradation through the coordinated action of three enzyme classes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s).
Ubr2 (Ubiquitin-protein ligase E3-alpha-2), also known as N-recognin-2, is a 1755 amino acid protein containing one UBR-type and one RING-type zinc finger. It functions as an E3 ubiquitin-protein ligase recognizing proteins with destabilizing N-terminal residues, leading to their ubiquitination and degradation. Mice lacking Ubr2 are infertile due to meiotic defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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