Thermo Fisher Scientific Cathepsin H/K/L/V Recombinant Rabbit Monoclonal Antibody (JM10-78)
Cathepsin H/K/L/V를 인식하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공. WB, IHC, ICC/IF, Flow Cytometry에 적합. HEK293 발현 시스템 사용, Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Thermo Fisher Scientific Cathepsin H/K/L/V Recombinant Rabbit Monoclonal Antibody (JM10-78)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM10-78 |
| Immunogen | Synthetic peptide within Human Cathepsin (aa 92–138) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809879 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
The Cathepsin family proteins are lysosomal cysteine proteinases that play major roles in intracellular protein catabolism. Their substrates include collagen, elastin, and alpha-1 protease inhibitor, which regulates neutrophil elastase activity.
These proteins are implicated in several pathological processes, such as:
- Myofibril necrosis in myopathies and myocardial ischemia
- Renal tubular response to proteinuria
Cathepsins are members of the peptidase C1 family, functioning as dimers composed of disulfide-linked heavy and light chains derived from a single precursor. Multiple alternatively spliced transcript variants are known for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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