Thermo Fisher Scientific CD27 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive control: K562
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death.
Expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 depend on proper ligation of antigen receptors, while CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals.

CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subpopulation of B cells. Activation of T cells via the TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane and release of its soluble 28–32 kDa form (sCD27), which can be detected in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker in acute and chronic B cell malignancies.

RgpA, a cysteine proteinase, can degrade CD27 and counteract T cell activation mediated by CD27 and CD70. CD27-binding protein (SIVA), a proapoptotic protein, binds to this receptor and may play a role in CD27-induced apoptosis.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.