Thermo Fisher Scientific Phospho-LDHA (Tyr10) Polyclonal Antibody
LDHA의 Tyr10 인산화 형태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 IHC(P)에서 검증됨. Human, Mouse, Rat 반응성. PBS/glycerol buffer에 보관하며, 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LDHA (Accession P00338), corresponding to amino acid residues around phosphorylated Tyr10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816873 |
Product Specific Information
This antibody detects endogenous levels of LDHA only when phosphorylated at Tyr10.
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes:
- LDHA (M or muscle form)
- LDHB (H or heart form)
- LDHC (X or testis form)
The three human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five different LDH isoenzymes based on the proportion of M and H chains in the tetrameric structure.
LDHA expression is hypoxia-inducible and regulated by HIF1α, while LDHB is often upregulated in cancers.
Mutations in the LDHA gene can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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