Thermo Fisher Scientific IL-21 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1:25,000–1:50,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Mouse IL-21.

Target Information

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th)17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which induces transcriptional activation by Bcl6. Similar to IFN-γ in Th1 and IL-4 in Th2 cells, IL-21 is critical for TFH cell development and effector function.

IL-21 plays a role in:

• T cell-dependent B cell differentiation into plasma and memory cells
• Stimulation of IgG production
• Induction of apoptotic signaling in naive B cells

In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4, and RORγt lead to upregulation of IL-23R, preparing Th17 cells for maturation and maintenance by IL-23. IL-21 also mediates downregulation of Foxp3.
IL-21 deficient mice are protected from chemically induced colitis due to inability to upregulate Th17-associated molecules, whereas elevated IL-21 levels are observed in colitis models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.