Thermo Fisher Scientific NOX4 Monoclonal Antibody (3H2C4)
인간 NOX4 단백질을 인식하는 Mouse monoclonal 항체입니다. Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능합니다. Protein G 정제, 1 mg/mL 농도, PBS 버퍼에 0.05% sodium azide 함유. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H2C4 |
| Immunogen | Purified recombinant fragment of human NOX4 (aa 210–310) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898498 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Oxygen sensing is essential for homeostasis in aerobic organisms. A phagocyte-type oxidase, similar to that in neutrophil granulocytes producing reactive oxygen species (ROS) for antimicrobial activity, is thought to function in the kidney as an oxygen sensor regulating erythropoietin synthesis in the renal cortex.
NOX4 acts as a redox messenger in intracellular signaling pathways contributing to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest NOX4 links the insulin receptor to ROS generation, enhancing insulin signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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