Thermo Fisher Scientific NOX4 Monoclonal Antibody (3H2G11)
Human NOX4 단백질을 인식하는 Mouse monoclonal antibody (Clone 3H2G11). Western blot, IHC, ICC, Flow cytometry, ELISA에 적합. Protein G로 정제된 액상 항체로 PBS에 0.05% sodium azide 포함. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H2G11 |
| Immunogen | Purified recombinant fragment of human NOX4 (aa 210–310) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898497 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Oxygen sensing is essential for homeostasis in all aerobic organisms. A phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes, with resultant antimicrobial activity, has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.
NOX4 functions as a redox messenger in the activation of intracellular signaling pathways involved in mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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