Thermo Fisher Scientific KCNK16 (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:600

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acid residues 75-88 of human KCNK16 (1st extracellular loop) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TALK-1,uniProtId:Q96T55-1,ncbiNodeId:9606,antigenRange:76-88,antigenLength:309,antigenImageFileName:PA5-111887_TALK-1_Q96T55-1_Rabbit.svg,antigenImageFileNamePDP:PA5-111887_TALK-1_Q96T55-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2857295

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Peptide sequence is: (C)EAWVKGV NPKGNST

Homology: Rat, mouse amino acid residues are identical.

Target Information

The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human Beta-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3). The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction. TALK-1, two-pore domain K+ (K2p 16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on Beta-cell CA2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH. TALK-1 is thus pH-sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.