Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Rabbit Monoclonal Antibody (WT1, 3477R)
Human WT1 단백질을 인식하는 Recombinant Rabbit Monoclonal Antibody로, IHC(P) 검증 완료. 고순도 Protein A/G 정제, 200 µg/mL 농도. PBS(pH 7.4) + 0.05% BSA, sodium azide 포함. 4°C 보관, 연구용 전용.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | WT1, 3477R |
| Immunogen | Synthetic peptide corresponding to WT1 residues (within aa1–100) of WT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells.
Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly.
WT1 and WIT2 are CC-TMD proteins that are structurally similar yet distinct from the WIP family, and are essential for RanGAP nuclear envelope association in plant and metazoan cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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