
Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI8H2), TrueMAB
Human UBA52 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화됨. 동결건조 형태로 제공되며 PBS 완충액에 8% 트레할로스 포함. 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 77–128 of human UBA52 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform additional desalting (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome.
The UBA52 gene encodes a fusion protein consisting of ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus.
When expressed in yeast, the protein is post-translationally processed to generate free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S ribosomal subunit, contains C4-type zinc finger domains, and is localized in the cytoplasm.
Pseudogenes derived from this gene are present in the genome. Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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