Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI7A8), TrueMAB
Human UBA52 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot 검증됨. Lyophilized 형태로 제공되며 PBS buffer에 trehalose 포함. 재구성 및 conjugation 실험에 적합하며 연구용으로 사용.
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Thermo Fisher Scientific UBA52 Monoclonal Antibody (OTI7A8), TrueMAB
Applications
- Western Blot (WB)
Tested Dilution
- 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7A8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 77–128 of human UBA52 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to reach approximately 1 mg/mL final concentration.
- For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ubiquitin is a highly conserved protein that targets cellular proteins for degradation by the 26S proteasome. The UBA52 gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed to generate free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S ribosomal subunit, belongs to the S27AE family, contains C4-type zinc finger domains, and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. Similar fusion proteins are also observed for ribosomal proteins L40 and S30.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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