Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 780, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Super Bright 780, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow series
• eFluor dyes (450, 506, 615, 660)
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine, PerCP-eFluor
• Super Bright 436, 600, 645, 702
• NovaFluor series
• Functional Grade
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as either CD8αβ heterodimer or CD8αα homodimer. CD8 binds MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal performance requires titration.

Fluorophore Information:
Super Bright 780 is excited at 405 nm and emits at 780 nm. Use a 780/60 bandpass filter or equivalent. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright conjugates, employ Super Bright Complete Staining Buffer (Product # SB-4401) to reduce non-specific polymer interactions.

Compensation Notes:
Higher compensation values may occur in the violet 450/50 channel when using UltraComp eBeads ([Product # 01-2222-42]) versus single-color stained cells. Compensation with cells is recommended. Similar effects may occur with AbC Total Antibody Compensation beads ([Product # A10497]).

Handling and Fixation:

• Light sensitive: protect from photo-oxidation.
• Samples can be stored in IC Fixation Buffer ([Product # 00-8222]) or 1-step Fix/Lyse Solution ([Product # 00-5333]) for up to 3 days at 4°C in the dark.

Laser Parameters:
Excitation: 405 nm
Emission: 780 nm
Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD8 is a type I transmembrane glycoprotein of the immunoglobulin family, functioning as a co-receptor with TCR for MHC class I molecules. It is expressed primarily on T cells, NK cells, monocytes, and dendritic cells. CD8 engagement leads to Lck recruitment, lymphokine production, and cytotoxic T lymphocyte activation. Differentiation of CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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