Himedia Nutrient Mixture F-12 Ham, Coon’s Modification w/ L-Glutamine w/o Sodium bicarbonate

Nutrient Mixture F-12 Ham, Coon’s Modification w/ L-Glutamine w/o Sodium bicarbonate

Details

Hams Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. F-12 is formulated for use with or without serum, depending on the type of cells being cultured. Hams Nutrient Mixture F12, Coon`s modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with Sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid.