Himedia Nutrient Mixture F-12 Ham, Coon’s Modification w/o L-Glutamine and Sodium bicarbonate
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Himedia
Himedia Nutrient Mixture F-10 Ham w/ L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate
Himedia
Himedia Medium 199 w/ Earle’s Salts, L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate
Himedia
Himedia Nutrient Mixture F-12 Ham, Coon’s Modification w/o L-Glutamine and Sodium bicarbonate
Nutrient Mixture F-12 Ham, Coon’s Modification w/o L-Glutamine and Sodium bicarbonate
Details
Hams Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham
s Nutrient Mixture F12, Coon`s modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid.
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