Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 488, eBioscience
Alexa Fluor 488로 표지된 Mouse IgG1 kappa 동형 대조항체로, IHC, ICC, Flow Cytometry에 사용 가능. 비특이적 결합 평가용으로 최적화되어 있으며, 4°C 암소 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633427 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- FITC
- Functional Grade
- Pacific Orange
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
Custom conjugation available upon request.
Product Specific Information
- Description: Monoclonal mouse IgG1 K immunoglobulin useful as an isotype control.
- Applications Reported: Suitable for immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
- Applications Tested: Pre-titrated and tested at 5 µL (1 µg) per test. A test is defined as the amount of antibody that stains a cell sample in 100 µL. Recommended cell count: 10⁵–10⁸ cells/test.
- Excitation / Emission: 488 nm / 519 nm (Blue Laser).
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a vital role in adaptive immunity, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It also regulates immune responses such as T cell activation and B cell inhibition. Widely used in research and diagnostics including ELISA, Western blot, and immunohistochemistry, as well as in therapeutic research like immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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