Thermo Fisher Scientific CD365 (Tim-1) Recombinant Rabbit Monoclonal Antibody (P365D), Alexa Fluor 488, eBioscience
인간 CD365(Tim-1/Kim-1/Havcr1)을 인식하는 재조합 토끼 단클론 항체. Alexa Fluor 488 형광 표지로 유세포분석(Flow Cytometry)에 최적화. Blue Laser(488 nm)에서 Excitation/Emission 499/520 nm. 단백질 A 정제, 4°C 암소 보관.
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Thermo Fisher Scientific CD365 (Tim-1) Recombinant Rabbit Monoclonal Antibody (P365D), Alexa Fluor 488
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | P365D |
| Immunogen | Protein corresponding to Human CD365 (aa22-aa129) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Protein A |
| Storage Buffer | Proprietary buffer / PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2848432 |
Additional Formats
- Unconjugated
- Alexa Fluor 647
- Custom conjugation available upon request
Product Specific Information
This P365D recombinant rabbit monoclonal antibody reacts with human CD365, also known as Tim-1, Kim-1, or Havcr1.
It has been pre-diluted and tested by flow cytometric analysis of 769P cells. Recommended usage is 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Post-manufacturing filtration: 0.2 µm.
Target Information
CD365 (Tim-1) is a type I surface glycoprotein with an extracellular N-terminal immunoglobulin V-like domain, a mucin domain, and a short cytoplasmic tail. The endogenous ligand is Tim-4.
CD365 acts as a receptor or co-receptor for multiple viruses, including hepatitis A/C, Ebola, Marburg, Dengue, Zika, West Nile, and Japanese encephalitis viruses, via phosphatidylserine binding.
Polymorphisms in the HAVCR1 gene are linked to infection susceptibility and disease severity.
CD365 is primarily expressed in epithelial cells and highly expressed in A549, 769-P, and Caco-2 cell lines. A soluble form also exists via metalloproteinase-mediated cleavage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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