Thermo Fisher Scientific CD365 (Tim-1) Recombinant Rabbit Monoclonal Antibody (P365D), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

P365D

Immunogen

Protein corresponding to Human CD365 (aa22-aa129) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KIM-1,uniProtId:Q96D42-1,ncbiNodeId:9606,antigenRange:22-129,antigenLength:364,antigenImageFileName:53-3658-42_KIM-1_Q96D42-1_Rabbit.svg,antigenImageFileNamePDP:53-3658-42_KIM-1_Q96D42-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Alexa Fluor 647
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Protein A

Storage buffer

proprietary buffer/PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2848432

Product Specific Information

Description: This P365D recombinant rabbit monoclonal antibody reacts with human CD365, which is also known as Tim-1, Kim-1, or Havcr1.

Applications Tested: This P365D antibody has been pre-diluted and tested by flow cytometric analysis of 769P cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail. The endogenous ligand for CD365 is Tim-4. In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus. The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes. Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity. CD365 has also been implicated in the T cell co-stimulation. CD365 is primarily expressed in epithelial cells. It is also highly expressed in A549, 769-P and Caco-2 and cell lines. CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.