Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme. This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).

Refer to BestProtocols®:
Protocol A – Two-step protocol for cytoplasmic intracellular proteins.

• Recommended use: ≤ 1.0 µg/test
• Test volume: 100 µL
• Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, the final step in the urea cycle. The resulting polyamines are vital for cell proliferation and detoxification of protein degradation byproducts.

By degrading arginine, Arginase 1 reduces nitric oxide synthesis by depriving NO synthase of its substrate.
It is expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells in both human and mouse.

• In humans: expressed in blood neutrophils, not in CCR3+ granulocytes
• In mice: marker of alternatively activated macrophages (M2a)
• Found in myeloid cells infiltrating tumors and in most hepatocellular carcinomas
• Defects cause argininemia, an autosomal recessive disorder with hyperammonemia

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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