Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 488, eBioscience
인간 및 마우스 Arginase 1을 인식하는 단클론 항체로, Alexa Fluor 488 형광 표지. Flow cytometry 및 면역염색에 적합. 488 nm 여기/519 nm 방출, Blue Laser 사용. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/test | View 25 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived recombinant mouse Arginase 1 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2734830 |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme. This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Refer to BestProtocols®:
Protocol A – Two-step protocol for cytoplasmic intracellular proteins.
- Recommended use: ≤ 1.0 µg/test
- Test volume: 100 µL
- Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, the final step in the urea cycle. The resulting polyamines are vital for cell proliferation and detoxification of protein degradation byproducts.
By degrading arginine, Arginase 1 reduces nitric oxide synthesis by depriving NO synthase of its substrate.
It is expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells in both human and mouse.
- In humans: expressed in blood neutrophils, not in CCR3+ granulocytes
- In mice: marker of alternatively activated macrophages (M2a)
- Found in myeloid cells infiltrating tumors and in most hepatocellular carcinomas
- Defects cause argininemia, an autosomal recessive disorder with hyperammonemia
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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