Thermo Fisher Scientific CD20 Recombinant Rabbit Monoclonal Antibody (PD00-02)
Thermo Fisher CD20 Recombinant Rabbit Monoclonal Antibody (PD00-02)는 인간 및 생쥐 시료에 반응하며, IHC(P) 및 IHC(PFA) 분석에 적합한 고순도 단일클론 항체입니다. HEK293 발현 시스템에서 생산되었으며, Protein A로 정제된 액상 형태로 안정적인 신호 검출을 제공합니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:1,000–1:2,000
Immunohistochemistry (PFA fixed) (IHC (PFA))
- Tested Dilution: 1:1,500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD00-02 |
| Immunogen | Synthetic peptide within Human CD20 aa 210–297 (Cytoplasmic) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3093779 |
Product Specific Information
- Positive Control: Human tonsil tissue, lymph nodes, spleen, small cell lung cancer, non-small cell lung cancer, prostate cancer, mouse spleen tissue
- Predicted Band Size: 33 kDa
- Subcellular Location: Cell membrane
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, as well as some T lymphocytes and follicular dendritic cells. It regulates transmembrane calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts and plays a role in B-cell receptor (BCR) signaling. It remains on the cell surface after antibody treatment, making it a valuable therapeutic target for B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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