Thermo Fisher Scientific EBP1 Recombinant Rabbit Monoclonal Antibody (JE35-48)
EBP1 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, Protein A로 정제되었습니다. 인간, 마우스, 랫트 시료에 반응하며, 세포질 및 핵에서 검출됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE35-48 |
| Immunogen | Synthetic peptide within Human EBP1 aa 325–374/394 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094156 |
Product Specific Information
- Positive control: K562 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, mouse cerebellum tissue, rat brain tissue, 293, NIH/3T3, PC-12
- Predicted band size: 44 kDa
- Subcellular Location: Cytoplasm, Nucleus
Target Information
The EBP1 gene encodes an RNA-binding protein involved in growth regulation. It is present in pre-ribosomal ribonucleoprotein complexes and may play a role in ribosome assembly and rRNA processing. EBP1 interacts with the cytoplasmic domain of the ErbB3 receptor, contributing to growth signal transduction. It also acts as a transcriptional co-repressor of androgen receptor-regulated and other cell cycle regulatory genes via histone deacetylase interactions. This protein has been linked to growth inhibition and differentiation induction in human cancer cells. Six pseudogenes have been identified on chromosomes 3, 6, 9, 18, 20, and X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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