Thermo Fisher Scientific CD150 Monoclonal Antibody (mShad150), FITC, eBioscience
마우스 CD150(SLAM) 단백질을 인식하는 단클론 항체로, FITC로 표지되어 Flow Cytometry 분석에 적합합니다. 활성화된 T세포 및 B세포에서 발현되는 CD150 검출에 유용하며, 0.5 µg/test 농도로 사용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- Publications: View 7 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, lambda |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mShad150 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11041521 |
Available Conjugates
- APC
- APC-eFluor 780
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The mShad150 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells (especially Th1) and B cells, and its expression is rapidly upregulated upon activation. It is also found on immature thymocytes and dendritic cells.
Signaling through SLAM in T cells induces proliferation and enhances interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells. The mShad150 antibody can stain the CD150⁺CD244⁻CD48⁻CD41⁻ population of pluripotent hematopoietic stem cells.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested on mouse splenocytes for flow cytometry. Recommended use ≤0.5 µg/test (100 µL final volume). Optimal titration advised.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule):
CD150 belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and some B cells. Expression is rapidly induced on naive T cells after activation. Activated B cells express multiple isoforms of SLAM (membrane-bound, soluble, cytoplasmic).
SLAM-SLAM interactions enhance B cell activation and differentiation. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain. Mutations in CD150 may be associated with X-linked lymphoproliferative disease (XLP).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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