Thermo Fisher Scientific CD150 Monoclonal Antibody (A12 (7D4)), FITC, eBioscience
인간 CD150(SLAM)을 인식하는 단클론 항체로, FITC 형광 표지되어 유세포분석(Flow Cytometry)에 최적화되었습니다. 활성화된 T 및 B 세포 분석에 적합하며, 488 nm 레이저에서 520 nm 방출을 가집니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (1 µg)/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A12 (7D4) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669579 |
Product Specific Information
Description:
The A12 monoclonal antibody reacts with human CD150, an approximately 70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and plays a role in activation of T and B cells.
Applications Reported:
This A12 (7D4) antibody has been reported for use in flow cytometric analysis.
Applications Tested:
The antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use is 5 µL (1 µg)/test in a final volume of 100 µL. The number of cells can range from 10⁵ to 10⁸ cells/test.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.
SLAM-SLAM homophilic interactions during B–B and B–T cell communication enhance proliferation and differentiation of activated B cells. CD150 also serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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