Thermo Fisher Scientific CD166 (ALCAM) Monoclonal Antibody (eBioALC48), FITC, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: -
• View 2 publications

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test
• View 4 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• PE
• Request custom conjugation

Product Specific Information

Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors. It is a type I transmembrane glycoprotein with five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail. ALCAM is involved in homotypic adhesion and heterotypic adhesion with its natural ligand CD6. A soluble isoform of ALCAM inhibits homophilic adhesive interactions.

ALCAM is expressed on activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells. It is also used as a marker for pluripotent stem cell isolation.

Applications Reported: This eBioALC48 antibody has been reported for use in flow cytometric analysis.

Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number: 10^5–10^8 cells/test. Antibody should be titrated for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The ALCAM (CD166) gene encodes a member of the immunoglobulin receptor subfamily with five Ig-like domains (VVC2C2C2) in the extracellular region. It binds to T-cell differentiation antigen CD6 and is involved in cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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