Thermo Fisher Scientific CD166 (ALCAM) Monoclonal Antibody (eBioALC48), FITC, eBioscience
CD166(ALCAM) 단일클론 항체(FITC 결합형)로 Flow Cytometry 및 IHC에 적합. 마우스 반응성, Rat IgG2a kappa 아이소타입. 0.5 mg/mL 농도, PBS 버퍼(0.09% sodium azide 포함). 4°C 암소 보관, 동결 금지.
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Applications
Immunohistochemistry (IHC)
- Tested Dilution: -
- View 2 publications
Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioALC48 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572479 |
Additional Formats
Product Specific Information
Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors. It is a type I transmembrane glycoprotein with five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail. ALCAM is involved in homotypic adhesion and heterotypic adhesion with its natural ligand CD6. A soluble isoform of ALCAM inhibits homophilic adhesive interactions.
ALCAM is expressed on activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells. It is also used as a marker for pluripotent stem cell isolation.
Applications Reported: This eBioALC48 antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number: 10^5–10^8 cells/test. Antibody should be titrated for optimal performance.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The ALCAM (CD166) gene encodes a member of the immunoglobulin receptor subfamily with five Ig-like domains (VVC2C2C2) in the extracellular region. It binds to T-cell differentiation antigen CD6 and is involved in cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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