Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), FITC, eBioscience

Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), FITC, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant UV 805
• eFluor 660
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The 14D3 monoclonal antibody reacts with human CD152 (CTLA-4), a protein structurally similar to CD28. CTLA-4 is expressed on activated T cells (and sometimes B cells) and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 and CD28 deliver opposing signals: CD28 acts as a costimulator, while CTLA-4 restricts T cell activation by inhibiting IL-2 secretion and proliferation. The cytoplasmic domain of CTLA-4 contains ER retention motifs, resulting in intracellular localization of newly synthesized CTLA-4 in response to TCR signaling.

The 14D3 antibody also recognizes rhesus monkey and shows inhibitory activity.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Use at 5 µL (0.125 µg) per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test).

Due to intracellular localization of CTLA-4, both surface and intracellular expression should be assessed for complete detection.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed mainly on CD4+ T cells. It binds CD80 and CD86 on B cells and dendritic cells with higher affinity than CD28.
Unlike CD28, which enhances T cell activation, CTLA4 inhibits T cell function. Intracellular CTLA4 is also found in regulatory T cells and is essential for their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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