Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), FITC, eBioscience
인간 및 원숭이 CD152(CTLA-4)를 인식하는 14D3 클론의 FITC 결합 단일클론 항체로, 활성화된 T세포 분석에 적합. Flow cytometry용으로 최적화되어 있으며, 498/517nm 형광 특성. PBS/BSA 버퍼에 보관하며 4°C 암소 저장.
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Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14D3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637118 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The 14D3 monoclonal antibody reacts with human CD152 (CTLA-4), a protein structurally similar to CD28. CTLA-4 is expressed on activated T cells (and sometimes B cells) and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 and CD28 deliver opposing signals: CD28 acts as a costimulator, while CTLA-4 restricts T cell activation by inhibiting IL-2 secretion and proliferation. The cytoplasmic domain of CTLA-4 contains ER retention motifs, resulting in intracellular localization of newly synthesized CTLA-4 in response to TCR signaling.
The 14D3 antibody also recognizes rhesus monkey and shows inhibitory activity.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Use at 5 µL (0.125 µg) per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test).
Due to intracellular localization of CTLA-4, both surface and intracellular expression should be assessed for complete detection.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed mainly on CD4+ T cells. It binds CD80 and CD86 on B cells and dendritic cells with higher affinity than CD28.
Unlike CD28, which enhances T cell activation, CTLA4 inhibits T cell function. Intracellular CTLA4 is also found in regulatory T cells and is essential for their activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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