Thermo Fisher Scientific Lamin B2 Polyclonal Antibody
Lamin B2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), IP에 사용 가능. Human 및 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보관되며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:40,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 221–500 of Lamin B2 (Uniprot ID: Q03252) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.73 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153954 |
Product Specific Information
- Recommended positive controls: H1299, HeLa, HepG2, 293T, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12
- Predicted reactivity: Rat (82%), Chicken (83%), Bovine (80%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins.
A-type lamins include lamin A, lamin C, and lamin Adel 10 (derived from the same gene by alternative splicing), while B-type lamins include lamin B1 and lamin B2 (from distinct genes).
Mutations in A-type lamins are associated with several dominant genetic disorders such as Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease, and Dunnigan-type familial partial lipodystrophy.
A-type lamins are expressed during cell differentiation and interact with chromatin, suggesting a role in regulating differential gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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