Thermo Fisher Scientific OGT Polyclonal Antibody
OGT 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. 고농도 액상 형태로 단기 4°C, 장기 -20°C 보관.
- 카탈로그번호
- PA522071
- 판매단위
- pk
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 223–502 of O-GlcNAc transferase (Uniprot ID: O15294) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.51 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152264 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, PC-12
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Pig (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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