Thermo Fisher Scientific Villin Polyclonal Antibody
Villin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC 등 다양한 실험에 적합. Human, Mouse, Rat 반응. 항원 친화 크로마토그래피로 정제되었으며, 고순도 PBS buffer에 보존. Villin 관련 세포골격 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment containing a sequence corresponding to amino acids 7–246 (P09327) of Villin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11155190 |
Product Specific Information
- Recommended positive controls: HepG2, mouse kidney, rat kidney
- Predicted reactivity: Mouse (89%), Rat (89%), Bovine (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush borders. It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner. Villin serves as a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in certain Merkel cell tumors and adenocarcinomas of the lung, prostate, ovary, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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