Thermo Fisher Scientific Phospho-APAF1 (Ser320) Polyclonal Antibody
APAF1 단백질의 Ser320 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot에 적합하며 Human, Mouse, Rat에서 반응. 고순도 액상 형태로 제공되며 -20°C 보관. 세포 사멸 연구용에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human APAF1 (Accession O14727), corresponding to amino acid residues around phosphorylated Ser320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816557 |
Product Specific Information
Antibody detects endogenous levels of APAF1 only when phosphorylated at Ser320.
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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