Thermo Fisher Scientific Cytokeratin 19 Monoclonal Antibody (BA17), eBioscience
사이토케라틴 19(CK19)에 특이적인 BA17 단일클론 항체로, 인간 및 마우스 시료에 반응. 면역조직화학, 면역세포화학, 웨스턴블롯 등에 사용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적 성능 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Cytokeratin 19 Monoclonal Antibody (BA17), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Flow Cytometry (Flow) | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BA17 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865509 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
Product Specific Information
This BA17 monoclonal antibody reacts with human cytokeratin 19 (CK19), a 44-kDa type I (acidic) intermediate filament protein lacking the non-alpha-helical tail domain found in other keratins. Cytokeratin 19 forms part of the cytoskeletal network that maintains cell and tissue integrity. It is expressed in a wide range of epithelial tissues and can be induced by vitamin A, SV40 transformation, and cancer. A soluble form generated by caspase 3 cleavage may indicate tumor metastasis. CK19 often forms heterodimers with cytokeratin 7.
Applications Reported: Suitable for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections and immunocytochemistry.
Applications Tested: Tested by immunocytochemistry of fixed and permeabilized MCF-7 cells at ≤10 µg/mL; optimal concentration should be titrated per assay.
Target Information
Cytokeratin 19 belongs to the intermediate filament protein family, characterized by biochemical diversity (20 polypeptides in human epithelial tissues). Molecular weights range from 40–68 kDa and isoelectric points from pH 4.9–7.8. CK19 expression is epithelium-type and differentiation-dependent, aiding in distinguishing epithelial malignancies. Cytokeratin antibodies assist in tumor differential diagnosis and are useful in cytopathology and flow cytometry. CK19 is not expressed in hepatocytes, making it a useful marker for identifying liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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