Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eBioscience
GFAP 단백질을 인식하는 GA5 클론 단일클론 항체로, 인간·마우스·랫 등 다양한 종에 반응. Western blot, IHC, ICC, Flow Cytometry 등에 사용 가능. 액상 형태, 고순도(>90%) 및 낮은 응집도(<10%)로 안정적 실험 재현성 제공.
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Thermo Fisher Scientific GFAP Monoclonal Antibody (GA5), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–5 µg/mL | View 5 publications |
| Immunohistochemistry (IHC) | – | View 26 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 5 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 9 publications |
| Flow Cytometry (Flow) | Assay-Dependent | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rabbit, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GA5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865501 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). GFAP is a 49-kDa type III intermediate filament protein expressed in neural tissues and distinguishes astrocytes from other glial cells during CNS development.
Three splice variants exist, with alpha-GFAP being the predominant form. GFAP can co-assemble with vimentin and nestin, though not required for filament assembly. Assembly depends on phosphorylation and dephosphorylation of the N-terminal domain.
Mutations in GFAP gene lead to Alexander disease, and GFAP is overexpressed in certain glial-derived tumors.
Applications Reported: Immunocytochemistry, Western blot, IHC (FFPE tissue), Flow cytometry
Applications Tested: Immunoblotting (mouse brain lysate), ICC (C6 cells), IHC (FFPE human tissue, low pH antigen retrieval)
Recommended Concentration: ≤5 µg/mL
Target Information
GFAP (Glial Fibrillary Acidic Protein) is a class III intermediate filament protein expressed in astrocytes and certain astroglia of the CNS, satellite cells, and non-myelinating Schwann cells. Neural stem cells also express GFAP.
GFAP antibodies serve as markers for astrocytic cells and are useful in identifying brain tumors derived from astrocytes. GFAP presence has also been reported in lens epithelium, Kupffer cells, salivary tumors, and erythrocytes.
Mutations in the GFAP gene cause Alexander disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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