Thermo Fisher Scientific HSPA4 Monoclonal Antibody (5A6)
HSP70 단백질을 검출하는 Mouse monoclonal antibody (Clone 5A6). Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. Human과 Mouse 시료에 반응하며, 고순도 정제된 액상 형태로 제공. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A6 |
| Immunogen | Purified recombinant fragment of human HSP70 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10984475 |
Product Specific Information
MA5-15578 targets HSP70 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.
The immunogen is a purified recombinant fragment of human HSP70 expressed in E. coli.
MA5-15578 detects HSP70, which has a predicted molecular weight of approximately 70 kDa.
Target Information
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75, and GRP78.
HSP70 expression is strongly induced in response to heat stress. HSP70 and HSC70 play key roles in cytosolic, endoplasmic reticulum, and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells.
GRP78 is localized in the endoplasmic reticulum, where it assists in the folding and translocation of nascent peptide chains.
GRP75 is restricted to the mitochondrial matrix and aids in the translocation and folding of polypeptide chains of both nuclear and mitochondrial origin.
Members of the HSP70 family act as force-generating motors, relying on ATP hydrolysis for their activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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