Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (4F8), APC
Nitrotryptophan 단백질을 특이적으로 인식하는 마우스 모노클로날 항체로 APC 형광 표지. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 6-Nitrotryptophan 변형 단백질 검출에 최적화되어 있으며, 높은 특이성과 민감도를 제공.
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Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (4F8), APC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F8 |
| Immunogen | Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45821 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 6-Nitrotryptophan modified proteins. Does not cross-react with 7-Ketocholesterol modified proteins.
Target Information
6-Nitrotryptophan (6-NO₂-Trp) is one of the most stable and major ONOO⁻-modified amino acids. Evidence suggests that nitrotryptophan plays a significant role in cellular regulation under nitrosative stress.
Reactive nitrogen species (RNS) are formed in various diseases such as atherosclerosis, inflammation, cancer, and neurodegenerative disorders.
Peroxynitrite (ONOO⁻) is a potent biological oxidant formed via rapid reaction between nitric oxide (NO) and superoxide (O₂⁻). It can induce nitration of aromatic amino acids such as Tyr, Trp, Phe, and His, leading to modulation of protein functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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