Thermo Fisher Scientific Dityrosine Monoclonal Antibody (7D4), APC
Dityrosine 단백질 변형을 특이적으로 인식하는 APC-conjugated mouse monoclonal antibody. Western blot, ICC/IF, ELISA에 사용 가능. 형광 특성 651/660 nm. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7D4 |
| Immunogen | Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932279 |
Additional Formats
- Unconjugated (MA5-27575)
- FITC (MA5-45826)
- PE (MA5-45828)
- PerCP (MA5-45827)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45825 was sufficient for detection of dityrosine in 1 µg of dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for dityrosine-modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine-modified proteins.
Target Information
Dityrosine may be a natural component of protein structure, a product of environmental stress, or a product of in vitro protein modification.
It is involved in diverse reactions that produce tyrosyl radicals, which in turn may couple to yield dityrosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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