Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (4F8), PerCP
6-Nitrotryptophan 변형 단백질을 특이적으로 인식하는 단클론 항체. PerCP 결합 형광 표지로 다양한 면역분석(WB, IHC, ICC, ELISA)에 적합. 482/675 nm 파장 특성. 연구용으로 nitrosative stress 관련 단백질 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F8 |
| Immunogen | Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45823 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 6-Nitrotryptophan modified proteins. Does not cross-react with 7-Ketocholesterol modified proteins.
Target Information
6-nitrotryptophan (6-NO2-Trp) is one of the most stable and major ONOO⁻-modified amino acids. Evidence suggests that nitrotryptophan plays a significant role in cellular regulation under nitrosative stress.
Reactive nitrogen species (RNS) are formed in diseases such as atherosclerosis, inflammation, cancer, and neurodegenerative disorders.
Peroxynitrite (ONOO⁻), a potent biological oxidant formed by the reaction between nitric oxide (NO) and superoxide (O₂⁻), can induce nitration of aromatic amino acids such as Tyr, Trp, Phe, and His, resulting in modulation of protein functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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