Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody (V9)
Vimentin 단백질을 인식하는 마우스 단클론 항체(V9)로, 중간엽 세포 마커 연구에 적합합니다. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, 다종 교차 반응성을 보입니다. 단백질 A/G로 정제된 액상 형태로, 보존제 무첨가 PBS 용액에 제공됩니다.
- 카탈로그번호
- 7431-MSM5-P1ABX
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg / 10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Horse, Cat, Human, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V9 |
| Immunogen | Porcine Lens (Target Family: Vimentin, UniProt ID: P02543-1, Antigen Range: 1–466) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to cytoskeletal reorganization that facilitates cholesterol transport to mitochondria, enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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